# Facility Health Data The facility-specific reports below contain a summary of cumulative results for the previous 18 months and details of the facility's most recent quarterly testing. A description of the surveillance program is provided in the [Health & Husbandry Statement.](https://hccmpub.med.harvard.edu/resource/summary-health-and-husbandry-statementpdf) [Details on the Pathogen test profile and schedule](https://hccmpub.med.harvard.edu/pathogen-test-profile-and-schedule) To request information not available on this site, email . ## Current Facility Health Data [### Facility Outbreak Summary ](/sites/g/files/omnuum8376/files/2026-03/Outbreak%20Summary.pdf) [### HSPH T1 2026 ](/sites/g/files/omnuum8376/files/2026-02/HSPH%20T1%2026.pdf) [### SGM Q4 2024 ](/sites/g/files/omnuum8376/files/hccm_public/files/sgm_q4_24.pdf) [### VSC T2 2026 ](/sites/g/files/omnuum8376/files/2026-06/VSC%20T2%2026.pdf) [### WAB T1 2026 ](/sites/g/files/omnuum8376/files/2026-05/WAB%20T1%2026.pdf) ## Programmatic Descriptions Open all sections Close all sections ### Health Surveillance Method - Mice expand\_more ## **Mice** Through Q1 2025, all mouse colony surveillance was conducted quarterly in all BL-1N animal rooms in HCCM-managed rodent facilities via dirty-bedding sentinels. Outbred sentinel mice (4-6 weeks of age) were purchased from an approved vendor (Taconic Farms or Charles River Laboratories) each quarter and housed two animals per cage, one cage per rack. Cages were changed weekly or bi-weekly, depending on the cage type and facility management plan. Sentinels received soiled bedding from a pooled sample of bedding taken from colony cages on their rack. Fifty per cent of sentinels’ cage bedding was soiled bedding from the colony animals under surveillance. Surveillance diagnostics were initially carried out on one sentinel animal per rack. Effective June 2025, all facilities transitioned from live dirty-bedding sentinels to a filter media sample exposed to dirty cage bedding collected from colony animals. The filter media is collected at the end of the surveillance period for PCR testing. One surveillance cage is seeded per rack. For each individual facility, cumulative health reports reflecting 18 months’ worth of surveillance testing will contain language documenting the date of the transition from live mice to filter-media sampling of dirty bedding. For all surveillance methods, the testing schedule is as follows: Serologic examination (sentinel mice through Q1 2025) or PCR of filter media (June 2025 onwards) for *Mycoplasma pulmonis*, Sendai Virus, Mouse Hepatitis Virus, Minute Virus of Mice, GDVII, Reo3, Pneumonia Virus of Mice, Mouse parvovirus, and Mouse Rotavirus (EDIM); internal parasites, fur mites, and C. bovis by PCR. In addition to the panel described above, a more expanded panel is run in the second trimester for mouse adenovirus 1 & 2, K virus, Polyoma virus, Lymphocytic Choriomeningitis virus (LCMV), and ectromelia virus. Unexpected clinical conditions in colony mice may prompt evaluation for the presence of other agents as necessary, such as respiratory or enteric bacterial pathogens (e.g. *Klebsiella pneumoniae, Streptococcus pyogenes, Pasteurella pneumotropica, Bordatella bronchiseptica; Salmonella enteriditis, Citrobacter freundii*). ### Health Surveillance Method - Rats expand\_more ## **Rats** Rat surveillance is conducted via a filter media sample exposed to dirty cage bedding collected from colony animals. The filter media is collected at the end of the surveillance period for PCR testing. One surveillance cage is seeded per rack. At each surveillance period the media is tested by PCR for *Mycoplasma pulmonis*, Sendai Virus, Pneumonia Virus of Mice, Sialodacryoadenitis or Rat Coronavirus, Kilham Rat Virus, and Toolan's H-1 virus, internal parasites, mites, and C. bovis. In addition to the panel described above, annual evaluation in the second trimester is performed for Cilia-associated respiratory bacillus, Hanta virus, Mouse Adenovirus 1 & 2, *Encephalitozoon cuniculi*, Lymphocytic Choriomeningitis virus (LCMV). ### Surveillance Program expand\_more Mice Rats ## Mice **T1/T3** Sendai virus, Pneumonia virus of mice, Mouse hepatitis virus, Minute virus of mice, GD-7 virus (Theiler's meningoencephalomyelitis virus), Reo-3 virus, Mouse parvovirus, Epizootic Diarrhea of Infant Mice (EDIM), Mouse Norovirus (not an excluded agent), *Mycoplasma pulmonis*, intestinal nematodes, fur mites, and C. bovis **T2** Above plus Lymphocytic choriomeningitis virus, mouse adenovirus, K virus, Polyoma virus and Ectromelia ## Rats **T1/T3** Sendai virus, Pneumonia virus of mice, Sialodacryoadenitis virus, Kilham rat virus, Toolan's H-1virus, Reo-3 virus, Rat parvovirus, Mycoplasma pulmonis, Pneumocystis carinii (not an excluded agent), intestinal nematodes, fur mites, and C. bovis **T2** Above plus GD-7 virus (Theiler's meningoencephalomyelitis virus), Lymphocytic choriomeningitis virus and Mouse adenovirus 1 & 2 ### Quarantine Program expand\_more Sort ##### Quarantine Program #### Species #### Agents Tested #### Programmatic Details **Mouse** PCR:Sendai virus, Pneumonia virus of mice,Mouse hepatitis virus, Minute virus of mice, GD-7 virus, Reo-3 virus, Mouse parvovirus (MPV 1-4), Epizootic Diarrhea of Infant Mice (EDIM), Lymphocytic choriomeningitis virus, Ectromelia virus, K virus, Mouse Norovirus (Not an excluded agent), Polyoma virus, Mouse adenovirus 1 & 2, Hanta Seoul, Hantaan virus, Cilia-associated Respiratory bacillus, *Mycoplasma pulmonis* Parasitology: Intestinal nematodes, fur mites (PCR). Quarantine is mandatory for animals arriving from non-approved suppliers. This is to ensure that animals introduced to specific pathogen free (SPF) colonies are also free of pathogens. Prophylactic Treatments: Imported animals are treated prophylactically with fenbendazole feed for nematodes and Selamectin topical treatments for ectoparasites (fur mites). Note: Hairless animals (i.e. hairless pups and nude mice) are not infested with fur mites and therefore are not treated with topical Selamectin. Assessment: Fecal samples and pelage samples are sent for PCR evaluation. Results: Data from the HCCM Diagnostic Laboratory and referral laboratories are assembled. Negative results will qualify animals for transfer out of quarantine to colony housing in the facility requested. As per prior arrangements stipulated in the quarantine process, positive results for excluded, untreatable pathogens will require humane sacrifice of the quarantined animals or removal of the quarantine lot by HCCM from HMS facilities to a commercial vendor for re-derivation. Pathogens for which effective treatment is available and implemented will be confined by HCCM and reevaluation with negative results will be required prior to release. **Rat** PCR: Sendai virus, Pneumonia virus of mice, Sialodacryoadenitis virus, Kilham rat virus, Toolan's H-1virus, GD-7 virus, Reo-3 virus, Rat parvovirus, Lymphocytic choriomeningitis virus, Mouse adenovirus 1 & 2, Hanta Seoul, Hantaan virus, Pneumocystis carinii (not an excluded agent), Cilia-associated respiratory bacillus, *Mycoplasma pulmonis* Parasitology: Intestinal nematodes, fur mites (PCR). As for mice.