Pathogen Test Profile and Schedule

Species

Biosafety level

Agents tested

Q1

Q2

Q3

Q4

Mouse rack sentinels receiving soiled bedding

BL1-N

Sendai virus, Pneumonia virus of mice, Mouse hepatitis virus, Minute virus of mice, GD-7 virus (Theiler's meningoencephalomyelitis virus), Reo-3 virus, Mouse parvovirus, Epizootic Diarrhea of Infant Mice (EDIM), Mouse Norovirus (Not an excluded agent),Mycoplasma pulmonis, Intestinal nematodes (PCR, fecal flotation & cellophane tape test), fur mites (PCR, microscopic exam of pelage pluck).

Yes

-

Yes

Yes

BL1-N

Above plus Lymphocytic choriomeningitis virus, mouse adenovirus, K virus, Polyoma virus and Ectromelia.

-

Yes

-

-

BL2-N

Sentinel program for BL2-N facilities is ended. Animals are obtained from barrier facilities by transfer or directly purhased from approved vendors. Quarterly surveillance data for times prior to September 2006 is available. Sendai virus, Pneumonia virus of mice, Mouse hepatitis virus, Minute virus of mice, GD-7 virus (Theiler's meningoencephalomyelitis virus), GD-7 virus, Reo-3 virus, Mouse parvovirus, EDIM, LCMV, Mouse Adenovirus (1 & 2), Ectromelia virus, K virus, Polyoma virus, Mycoplasma pulmonis

No

No

No

No

Rat rack sentinels recieving soiled bedding

BL1-N

Sendai virus, Pneumonia virus of mice, Sialodacryoadenitis virus, Kilham rat virus, Toolan's H-1virus, Reo-3 virus, Rat parvovirus, Mycoplasma pulmonis,Pneumocystis carinii (not an excluded agent), Intestinal nematodes (fecal flotation & cellophane tape test), fur mites (microscopic exam of pelage pluck).

Yes

-

Yes

Yes

BL1-N

Above plus GD-7 virus (Theiler's meningoencephalomyelitis virus), Lymphocytic choriomeningitis virus and Mouse adenovirus 1 & 2.

-

Yes

-

-

BL2-N

Sentinel program for BL2-N facilities is ended. Animals are obtained from barrier facilities by transfer or directly purhased from approved vendors. Sendai virus, Pneumonia virus of mice, Sialodacryoadenitis virus, Kilham rat virus, Toolan's H-1 virus, Reo-3 virus, Rat parvovirus, GD-7 virus, Lymphocytic choriomeningitis virus and Mouse adenovirus 1 & 2, Mycoplasma pulmonis

No

No

No

No

BL1-N

Sendai virus, SV-5, Pneumonia virus of mice, Reo-3 virus, Lymphocytic choriomeningitis virus

Yes

-

Yes

Yes

Above plus Encephalitozoon cuniculi

-

Yes

-

-

Quarantine Program

Species

Agents Tested

Programmatic Details

Colony Mouse

PCR (Pelage, oral swabs and fecal sample):Sendai virus, Pneumonia virus of mice,Mouse hepatitis virus, Minute virus of mice, GD-7 virus, Reo-3 virus, Mouse parvovirus (MPV 1-4), Epizootic Diarrhea of Infant Mice (EDIM), Lymphocytic choriomeningitis virus, Ectromelia virus, K virus, Mouse Norovirus (Not an excluded agent), Polyoma virus, Mouse adenovirus 1 & 2, Hanta Seoul, Hantaan virus, Cilia-associated Respiratory bacillus, Mycoplasma pulmonis

Parasitology: Intestinal nematodes, fur mites (PCR).

Quarantine is mandatory for animals arriving from non-approved suppliers. This is to ensure that animals introduced to specific pathogen free (SPF) colonies are also free of pathogens.

Prophylactic Treatments: Imported animals are treated prophylactically with fenbendazole feed for nematodes and Selamectin topical treatments for ectoparasites (fur mites).

Note: Hairless animals (i.e. hairless pups and nude mice) are not infested with fur mites and therefore are not treated with topical Selamectin.

Assessment: Fecal samples and pelage samples are sent for PCR evaluation.

Results: Data from the HCCM Diagnostic Laboratory and referral laboratories are assembled. Negative results will qualify animals for transfer out of quarantine to colony housing in the facility requested. As per prior arrangements stipulated in the quarantine process, positive results for excluded, untreatable pathogens will require humane sacrifice of the quarantined animals or removal of the quarantine lot by HCCM from HMS facilities to a commercial vendor for re-derivation.

Pathogens for which effective treatment is available and implemented will be confined by HCCM and reevaluation with negative results will be required prior to release.

Rat

PCR (Pelage, oral swabs and fecal sample): Sendai virus, Pneumonia virus of mice, Sialodacryoadenitis virus, Kilham rat virus, Toolan's H-1virus, GD-7 virus, Reo-3 virus, Rat parvovirus,Lymphocytic choriomeningitis virus, Mouse adenovirus 1 & 2, Hanta Seoul, Hantaan virus, Pneumocystis carinii (not an excluded agent), Cilia-associated respiratory bacillus, Mycoplasma pulmonis

Parasitology: Intestinal nematodes, fur mites (PCR).

As for mice.